Amyloid Precursor Protein (APP) Rabbit Polyclonal Antibody

CAT#: AP06009PU-N

Amyloid Precursor Protein (APP) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of APP/β-Amyloid antibody at 1/500 dilution Lane 1: MCF-7 cell lysate Lane 2: sp2/0 cell lysate Lane 3: H9C2 cell lysate

• 

  Immunohistochemical analysis using Amyloid beta A4 / APP antibody in Paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 701-750 of Human APP/β-Amyloid.
• Specificity: This antibody detects endogenous levels of Amyloid beta A4 protein / APP (region surrounding Glu737).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pur; by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 70 to140 kDa
• Gene Name: amyloid beta precursor protein
• Database Link:
  Entrez Gene 351 Human
  UniProt ID P05067
• Background: Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the β-Amyloid and Amyloid A4 proteins, which are present in human platelets. Amyloid deposition is associated with type II diabetes, Down syndrome and a variety of neurological disorders, including Alzheimers disease. The Amyloid precursor protein (APP) undergoes alternative splicing, resulting in several isoforms. Proteolytic cleavage of APP leads to the formation of the Amyloid β/A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimers patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimers disease patients.
• Synonyms: Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebral vascular amyloid peptide, CVAP