CCK4 (PTK7) (N-term) Rabbit Polyclonal Antibody

CAT#: AP05710PU-N

CCK4 (PTK7) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of human (A) mouse (B) and Rat (C) colon lysates probed with Rabbit anti Human PTK7 at 1 ug/ml

• 

  Formalin fixed, paraffin embedded human colon stained with Rabbit anti Human PTK7 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 0.5 - 1.0 µg/ml; detects a band of approximately 86kDa in colon tissue lysates.
  Immunohistochemistry on paraffin sections (requires antigen retrieval using heat treatment prior to staining).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A 15 amino acid peptide located near the N-terminal region of human PTK7.
• Specificity: This antibody recognises Protein tyrosine kinase-like 7 (PTK7).
• Formulation: Phosphate buffered saline containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine kinase 7 (inactive)
• Database Link:
  Entrez Gene 5754 Human
  UniProt ID Q13308
• Background: PTK7 is a member of the receptor tyrosine kinase family, otherwise known as Colon Carcinoma Kinase 4 (CCK4). PTK7 is highly expressed in the kidney, liver, lung, pancreas and melanocytes with weaker expression being detected in the thyroid gland, ovaries, brain, heart and skeletal muscle.
  
  PTK7 may play a role in embryonic development. It is thought to function as a cell adhesion molecule and as a novel regulator of the planar cell polarity (PCP) pathway in vertebrates. Studies also suggest that PTK7 may be involved in the tumour progression. A loss of PTK7 expression has been observed in melanoma cell lines, whereas increased levels of PTK7 have been detected in high-throughput acute myeloid leukaemia samples. In addition, PTK7 is not expressed in normal colon but has been detected in colon carcinomas and as such is a potential marker for tumour progression.
• Synonyms: Tyrosine-protein kinase-like 7, Colon carcinoma kinase 4, CCK-4