BIK (N-term) Rabbit Polyclonal Antibody

CAT#: AP05587PU-N

BIK (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of whole cell lysate from Jurkat, human T-cell leukemia cells probed with Rabbit anti Human BIK at 1 (A) and 2 (B) ug/ml

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  Immunocytochemical staining of Jurkat cells with Rabbit anti Human BIK

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1 - 2 µg/ml. Detects a band of approximately 18kDa in Jurkat cell lysates.
  Immunocytochemistry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: 15 amino acid peptide from near the amino terminus of human Bik.
• Specificity: This antibody detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: BCL2 interacting killer
• Database Link:
  Entrez Gene 638 Human
  UniProt ID Q13323
• Background: Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis.
  Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.
• Synonyms: Bcl-2-interacting killer, NBK, BP4, BIP1