Collagen I (COL1A1) (+ type III) Rabbit Polyclonal Antibody

CAT#: AP05393PU-N

Collagen I (COL1A1) (+ type III) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: ELISA: 1/500-1/2000.
  Immunofluorescence: 1/10-1/40.
  Immunohistochemistry on Frozen Sections: 1/10-1/40.
• Reactivities: Human, Porcine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Porcine Collagen, types 1 and 3 from skin
• Specificity: This antibody reacts with native and heat denatured Porcine Collagen type 1 and 3.
  Less than 0.5% reactivity is observed with Porcine Albumin and Immunoglobulins.
• Formulation: Mannitol, dextran and salts.
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore the vial (50 µg) with 500 µl of sterile distilled water or saline.
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type I alpha 1
• Database Link:
  Entrez Gene 1277 Human
  UniProt ID P02452
• Background: Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
  Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion