RIT2 Rabbit Polyclonal Antibody

CAT#: AP05116PU-N

RIT2 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: detects recombinant Rin protein expressed in HEK-293 cells; Shows minimal cross-reactivity with Rit protein and other Ras proteins.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: His-tagged recombinant human Rin protein
• Specificity: This antibody reacts to RIT2 (Rin).
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Ras like without CAAX 2
• Database Link:
  Entrez Gene 6014 Human
  UniProt ID Q99578
• Background: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.
• Synonyms: RIN, ROC2