Acid Phosphatase (ACP1) Sheep Polyclonal Antibody

CAT#: AP05058PU-N

Acid Phosphatase (ACP1) sheep polyclonal antibody, Purified

Product Images

CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and immunoprecipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse antisheep monoclonal antibody.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: Western Blot.
  Immunoprecipitation: 1-10 µg/ml.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The Human protein, HCPTPA was subcloned into pRSET to express a His6 N-terminal fusion protein.
  This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
• Specificity: This antibody reacts to LMW Protein Tyrosine Phosphatase.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.08% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulfate Precipitation
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: acid phosphatase 1, soluble
• Database Link:
  Entrez Gene 52 Human
  UniProt ID P24666
• Background: Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinylphosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1) 2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.
• Synonyms: Adipocyte acid phosphatase, Red cell acid phosphatase 1, HAAP