Orf1 Rabbit Polyclonal Antibody

CAT#: AP03009PU-N

Orf1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blotting analysis (reducing conditions) of recombinant protein Orf1 in cell lysate of Orf1-transfected E. coli using polyclonal anti-Orf1 antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Suitable for Western Blotting under Reducing conditions (0.2-1 μg/ml).
• Reactivities: Neisseria meningitidis
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
• Specificity: The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Protein-A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.