IKB epsilon (NFKBIE) Rabbit Polyclonal Antibody

CAT#: AP02784PU-N

IKB epsilon (NFKBIE) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extract from 293 cells, untreated or treated with TNF-a (20ng/ml, 15min), using IkB-?µ antibody (Lane 1 and 2) and IkB-?µ (PSer22) antibody (Lane 3 and 4).

• 

  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-?µ antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1:500~1:1000.
  Immunohistochemistry: 1:50~1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human IkB-ε around the phosphorylation site of serine 22 ( I-E-SP-L-R).
• Specificity: IkB-ε antibody detects endogenous levels of total IkB-ε protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: NFKB inhibitor epsilon
• Database Link:
  Entrez Gene 4794 Human
  UniProt ID O00221
• Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.
• Synonyms: I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon