Cortactin Rabbit Polyclonal Antibody

CAT#: AP02764PU-S

Cortactin rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin antibody.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1:50-1:100.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
• Specificity: Cortactin antibody detects endogenous levels of total Cortactin protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cortactin
• Database Link:
  Entrez Gene 13043 Mouse
  UniProt ID Q60598
• Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
• Synonyms: CTTN, EMS1, Amplaxin, Oncogene EMS1