EIF4E Rabbit Polyclonal Antibody
Other products for "EIF4E"
Specifications
Product Data | |
Applications | IF, IHC, WB |
Recommended Dilution | Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100 - 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100. |
Reactivities | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-Sp-T-T). |
Specificity | This antibody detects endogenous levels of total elF4E protein. |
Formulation | PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Affinity chromatography |
Conjugation | Unconjugated |
Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | eukaryotic translation initiation factor 4E |
Database Link | |
Background | eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk. |
Synonyms | eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F |
Reference Data |
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