Ephrin B2 (EFNB2) Rabbit Polyclonal Antibody

CAT#: AP02708PU-S

Ephrin B2 (EFNB2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HT29 cells using Epherin-B2 antibody

• 

  Immunofluorescence staining of methanol-fixed HeLa cells using Ephrin-B2 antibody (Red)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide Sequence around amino acids 328~332 (N-I-Y-Y-K) derived from Human Ephrin-B2.
• Specificity: This antibody AP02708PU detects endogenous levels of total Ephrin-B2 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ephrin B2
• Database Link:
  Entrez Gene 1948 Human
  UniProt ID P52799
• Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins.
  The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.
• Synonyms: EFNB2, EPLG5, HTKL, LERK5, HTK-L, LERK-5, HTK ligand
• Note: Molecular Weight: 37 kDa