MEK1 (MAP2K1) Rabbit Polyclonal Antibody

CAT#: AP02693PU-N

MEK1 (MAP2K1) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from Jurkat cells using MEK1 antibody (Lane 1 and 2) and MEK1 (phospho-Ser221) antibody (Lane 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Suitable for use in in Western blot (1:500-1:1000) and Immunohistochemistry (1:50-1:100).
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-Sp-F-V).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  MEK1 antibody AP02693PU detects endogenous levels of total MEK1 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: mitogen-activated protein kinase kinase 1
• Database Link:
  Entrez Gene 5604 Human
  UniProt ID Q02750
• Background: MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. MEK1 is activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
• Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1