HDAC2 Rabbit Polyclonal Antibody

CAT#: AP02667PU-N

HDAC2 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from HT-29 cells untreated or treated with UV (20min), using HDAC2 antibody (Lane 1 and 2) and HDAC2 (Phospho-Ser394) antibody (Lane 3 and 4).

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  Figure 3 Western Blot analysis of extracts from 293 and HeLa cells using HDAC2 antibody

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HDAC2 antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 392~396 (E-D-S-G-D) derived from Human HDAC2.
• Specificity: This antibody detects endogenous levels of total HDAC2 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: histone deacetylase 2
• Database Link:
  Entrez Gene 3066 Human
  UniProt ID Q92769
• Background: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
• Synonyms: Histone deacetylase 2, HD2
• Note: Molecular Weight: 60 kDa