MYOD1 Rabbit Polyclonal Antibody

CAT#: AP02653PU-S

MYOD1 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from HeLa cells using MyoD antibody. (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking overnight.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 198~202 (A-S-S-P-R)
  derived from Human MyoD.
• Specificity: This antibody detects endogenous levels of total MyoD protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: myogenic differentiation 1
• Database Link:
  Entrez Gene 4654 Human
  UniProt ID P15172
• Background: MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
• Synonyms: MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3, BHLHC1
• Note: Molecular Weight: 40 kDa