G3BP (G3BP1) Rabbit Polyclonal Antibody

CAT#: AP02635PU-S

G3BP (G3BP1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from 293 cells using G3BP-1 antibody (Lane 1 and 2) and G3BP-1 (pSer232) antibody (Lane 3 and 4).

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1:500~1000.
  Immunohistochemsistry: 1:50~1:100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).
• Specificity: G3BP-1 antibody detects endogenous levels of total G3BP-1 protein.
• Formulation: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: G3BP stress granule assembly factor 1
• Database Link:
  Entrez Gene 10146 Human
  UniProt ID Q13283
• Background: G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
• Synonyms: G3BP-1, hDH VIII