Estrogen Receptor 1 (ESR1) Rabbit Polyclonal Antibody

CAT#: AP02609PU-N

Estrogen Receptor 1 (ESR1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from MCF cells using Estrogen Receptor-alpha antibody and the same antibody preincubated with blocking peptide

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a antibody.

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  Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-alpha antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide around amino acids 165~169 (L-A-S-T-N) derived from Human Estrogen Receptor-alpha
• Specificity: This antibody detects endogenous levels of total Estrogen Receptor-alpha protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66 kDa
• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.
  Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1