c-Myc (MYC) Rabbit Polyclonal Antibody

CAT#: AP02577PU-N

c-Myc (MYC) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HT-29 cells treated with UV (20min), using Myc antibody (Lane 1 and 2) and Myc pThr358 antibody ( Lane 3 and 4).

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Myc antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot (1/500~1/1000)
  Immunohistochemistry (1/50~1/100).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Myc around the phosphorylation site of threonine 358 (R-R-TP-H-N).
• Specificity: This antibody detects endogenous levels of total Myc protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
• Database Link:
  Entrez Gene 4609 Human
  UniProt ID P01106
• Background: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
• Synonyms: Transcription factor p64, BHLHE39