PTPN11 / PTP2C pTyr542 Rabbit Polyclonal Antibody

CAT#: AP02543PU-N

PTPN11 / PTP2C pTyr542 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from NIH/3T3 treated with EGF (200 ng/ml, 30 min) or C6 cells treated with Sorbitol (0.4 M, 30 min), using SHP-2 (Phospho-Tyr542) antibody (Lane 1, 2 and 3).

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SHP-2 (Phospho-Tyr542) antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1:500~1:1000.
  Immunohistochemistry: 1:50~1:100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human SHP-2 around the phosphorylation site of tyrosine 542 (H-E-YP-T-N).
• Specificity: The antibody detects endogenous levels of SHP only when phosphorylated at tyrosine 542.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine phosphatase, non-receptor type 11
• Database Link:
  Entrez Gene 5781 Human
  UniProt ID Q06124
• Synonyms: SHPTP2, PTP-1D, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3