GJA1 pSer368 Rabbit Polyclonal Antibody

CAT#: AP02508PU-S

GJA1 pSer368 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from HeLa (Line 1, 4, 7 and 10), K562 (Line 2, 5, 8 and 11) and 293 (Line 3, 6, 9 and 12) cells, untreated or treated with PMA (1?M 30min), using Connexin-43 antibody (Lane 1, 2, 3, 4, 5 and 6) and Connexin-43 (pSer367) antibody (Lane 7, 8, 9, 10, 11 and 12).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin43 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500~1/1000.
  Immunohistochemistry: 1/50~1/100 (Suitable for Paraffin Embedded Sections).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Connexin-43 around the phosphorylation site of serine 367 (R-A-SP-S-R).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
  The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.This antibody detects endogenous levels of Connexin43 only when phosphorylated at serine 367.
• Formulation: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG fractiion.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: gap junction protein alpha 1
• Database Link:
  Entrez Gene 2697 Human
  UniProt ID P17302
• Background: GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.
• Synonyms: Gap junction alpha-1 protein, Connexin-43, GJAL