EIF4E pSer209 Rabbit Polyclonal Antibody

CAT#: AP02491PU-N

EIF4E pSer209 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extract from NIH/3T3 cells untreated or treated with 10% serum (15min), using elF4E antibody (Lane 1 and 2) and elF4E pSer209 antibody (Line 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E pSer209 antibody

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  Figure 3. Immunofluorescence staining of methanol-fixed MCF7 cells using elF4E pSer209 antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
• Specificity: This antibody detects endogenous levels of elF4E only when phosphorylated at Serine 209.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  Entrez Gene 1977 Human
  UniProt ID P06730
• Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
• Synonyms: eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F