EIF4EBP1 pThr45 Rabbit Polyclonal Antibody

CAT#: AP02482PU-S

EIF4EBP1 pThr45 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min), using 4E-BP1 (pThr45) antibody (Lane 3 and 4).

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1:500~1:1000.
  Immunohistochemistry: 1:50~1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
• Specificity: 4E-BP1 antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
• Formulation: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  Entrez Gene 1978 Human
  UniProt ID Q13541
• Synonyms: PHAS-I, PHAS-1, PHAS1