NF-kB p65 (RELA) pSer529 Rabbit Polyclonal Antibody

CAT#: AP02476PU-S

NF-kB p65 (RELA) pSer529 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kB p65 antibody and NF-kB p65 pSer529 antibody (Line 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-kB p65 pSer529 antibody.

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  Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using NF-kB p65 pSer529 antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D).
• Specificity: This antibody detects endogenous levels of NF-κB p65 protein only when phosphorylated at serine 529.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  Entrez Gene 5970 Human
  UniProt ID Q04206
• Background: NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators.
  NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65.
  NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3.
  Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
• Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3