14-3-3 zeta (YWHAZ) pSer58 Rabbit Polyclonal Antibody

CAT#: AP02457PU-S

14-3-3 zeta (YWHAZ) pSer58 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-alpha (20 ng/ml, 5min), using 14-3-3 zeta antibody (lane 1 and 2) and 14-3-3 zeta (Phospho-Ser58) antibody (lane 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta (Phospho-Ser58) antibody.

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  Figure 3. Immunofluorescence staining of methanol-fixed Hela cells using using 14-3-3 zeta (Phospho-Ser58) antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Note: Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide Sequence around the phosphorylation site of Serine 58 (R-S-Sp-W-R) derived from Human 14-3-3 zeta.
• Specificity: This antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Serine 58.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  Entrez Gene 7534 Human
  UniProt ID P63104
• Synonyms: YWHAZ, KCIP-1
• Note: Molecular Weight: 28 kDa