ZAP70 pTyr493 Rabbit Polyclonal Antibody

CAT#: AP02442PU-S

ZAP70 pTyr493 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from JK cells untreated or treated with H2O2 (2mM 3min) using Zap-70 pTyr493 antibody.

• 

  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Zap-70 (phospho-Tyr493) antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot 1/500-1/1000).
  Immunohistochemistry on Paraffin-Embedded Sections (1/50-1/100).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-Yp-T-A).
• Specificity: This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 493.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: zeta chain of T cell receptor associated protein kinase 70kDa
• Database Link:
  Entrez Gene 7535 Human
  UniProt ID P43403
• Background: ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
• Synonyms: ZAP-70, ZAP 70, SRK