VEGF Receptor 2 (KDR) pTyr1214 Rabbit Polyclonal Antibody

CAT#: AP02383PU-N

VEGF Receptor 2 (KDR) pTyr1214 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from SKOV3 cells using VEGFR2 (pTyr1214) antibody (Lane 1) and the same antibody preincubated with blocking peptide (Lane 2)

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using VEGFR2 (phospho-Tyr1214) antibody.

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  Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2 (pTyr1214) antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1214 (F-H-YP-D-N).
• Specificity: VEGFR2 (phospho-Tyr1214) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1214.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: kinase insert domain receptor
• Database Link:
  Entrez Gene 3791 Human
  UniProt ID P35968
• Background: VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
• Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2