PTEN pSer380/pThr382/383 Rabbit Polyclonal Antibody

CAT#: AP02355PU-S

PTEN pSer380/pThr382/383 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extract from HeLa cells using PTEN (pSer380/pThr382/pThr383) antibody and the same antibody preincubated with blocking peptide

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (pSer380/pThr382/pThr383) antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fixed Hela cells using PTEN (pSer380/pThr382/pThr383) antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 -
  1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382/383 (R-Y-SP-D-TP-TP-D-S).
• Specificity: PTEN (phospho-Ser380/Thr382/Thr383) antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382/383.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: phosphatase and tensin homolog
• Database Link:
  Entrez Gene 5728 Human
  UniProt ID P60484
• Background: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
• Synonyms: MMAC1, TEP1