STAT3 pSer727 Rabbit Polyclonal Antibody

CAT#: AP02345PU-N

STAT3 pSer727 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from Hela and HepG2 cells untreated or treated with PMA using STAT3 )pSer727) Antibody

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using STAT3 (pSer727) antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fixed Hela cells using STAT3 (pSer727) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
• Specificity: STAT3 (phospho-Ser727) antibody detects endogenous levels of STAT3 only when phosphorylated at serine 727.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  Entrez Gene 6774 Human
  UniProt ID P40763
• Synonyms: STAT-3, Acute-phase response factor, APRF