eNOS (NOS3) pThr494 Rabbit Polyclonal Antibody

CAT#: AP01888PU-N

eNOS (NOS3) pThr494 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NOS3 (pThr494) antibody (Cat.-No.: AP01888PU-N) in extracts from HepG2 cells.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-NOS3 protein when phosphorylated at Thr494.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 140 kDa
• Gene Name: nitric oxide synthase 3
• Database Link:
  Entrez Gene 4846 Human
  UniProt ID P29474
• Background: Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS