Frizzled 2 (FZD2) Rabbit Polyclonal Antibody

CAT#: AP01425PU-N

Frizzled 2 (FZD2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Frizzled-2 antibody in extracts from HeLa cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluoresence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 208-260 of Human Frizzled-2.
• Specificity: This antibody detects endogenous levels of Frizzled-2 protein. (region surrounding Glu240)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~65 kDa
• Gene Name: frizzled class receptor 2
• Database Link:
  Entrez Gene 2535 Human
  UniProt ID Q14332
• Background: The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-XXX-Val motif. The proteins in this family function as receptors for Wnt and are generally coupled to G proteins. Frizzleds are members of the G protein-coupled receptor superfamily. Frizzled-2 is expressed in the fetal kidney and lung and in the adult ovary and colon. Frizzled-2 mediates the Wnt/cGMP/Ca2+ pathway. It binds Wnt proteins and signals by activating the release of stored calcium. Frizzled-2 expression is regulated by Angiotensin II. Activated frizzled-2 suppresses the activity of protein kinase G, and activates NFAT-dependent transcription, the phosphatidylinositol pathway and calcium sensitive enzymes.
• Synonyms: Fz-2, hFz2, FzE2