TPX2 Rabbit Polyclonal Antibody

CAT#: AP01372PU-N

TPX2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of TPX2 antibody (Cat.-No.: AP01372PU-N) in extracts from Jurkat.

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  Immunohistochemistry (IHC) analyzes of TPX2 antibody (Cat.-No.: AP01372PU-N) in paraffin-embedded human brain tissue

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: TPX2 antibody detects endogenous levels of TPX2 protein. (region surrounding Gln330)
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: TPX2, microtubule nucleation factor
• Database Link:
  Entrez Gene 22974 Human
  UniProt ID Q9ULW0
• Background: TPX2 (targeting protein for Xklp2) is a microtubule-associated protein involved in targeting the motor protein Xklp2 to microtubules. Ran-GTP activates TPX2 for the chromatin-induced microbutule assembly during M phase.
  Aurora-A kinase associates with TPX2 at the spindle apparatus and may regulate TPX2 via phosphorylation during the spindle assembly. TPX2 appears to play a structural role in spindle formation. TPX2 activates Eg2 in a microtubuledependent manner by stimulating the phosphorylation and kinase activity of Eg2. TPX2 is inactivated by binding to importin α, a nuclear import factor. Finally, the suppression of TPX2 with RNA interference causes defects in microtubule organization during mitosis.
• Synonyms: DIL-2, C20orf1, C20orf2, HCA519