Integrin alpha 4 (ITGA4) Rabbit Polyclonal Antibody

CAT#: AP01224PU-N

Integrin alpha 4 (ITGA4) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ITGA4 antibody in extracts from Jurkat cells treated with PMA 125ng/ml.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 1000-1032 of Human Integrin α4.
• Specificity: This antibody detects endogenous levels of Integrin alpha 4 (ITGA4/CD49d) protein. (region surrounding asn1023)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~115 kDa
• Gene Name: integrin subunit alpha 4
• Database Link:
  Entrez Gene 3676 Human
  UniProt ID P13612
• Background: Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.
• Synonyms: Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4