IL31 Rabbit Polyclonal Antibody

CAT#: AP01149PU-S

Reviews ()
Write a review

IL31 rabbit polyclonal antibody, Aff - Purified


Product Datasheet for 'AP01149PU-S'


USD 260.00


Availability*
2 Weeks

Size
    • 50 ug


Product images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Indirect ELISA: To detect  Human IL-31 (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.
Sandwich ELISA: To detect  Human IL-31 (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human IL-31 (Cat.-No. AP01149BT)as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-31.
Western blot: To detect Human IL-31 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (> 98%) E.coli derived recombinant Human IL-31
Specificity This antibody detects Human Interleukin-31.
Formulation PBS, pH 7.2
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Immunoaffinity Chromatography
Background Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia and skin lesions, and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.
Synonyms IL-31
Note Centrifuge vial prior to opening.
Reference Data
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Antibody Performance Guarantee banner
ANTIBODY VALIDATION 101
Clone ID reveals the Source of Monoclonal Antibodies