NOTCH1 (20-216,Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: S253-32]

CAT#: AM60030PU-N

NOTCH1 (20-216,Extracell. Dom.) mouse monoclonal antibody, clone S253-32, Purified

Specifications

Product Data

• Clone Name: S253-32
• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/1000.
  1 μg/ml of this antibody was sufficient for detection of NOTCH1 in 20μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG-HRP as the secondary antibody.
  Immunocytochemistry.
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Fusion protein, amino acids 20- 216 (extracellular N-terminus, EGFlike domains 1-5) of mouse NOTCH1
• Specificity: This antibody detects NOTCH1 (aa20-216); >270kDa, ~120kDa and small fragments due to proteolysis.
  Does not cross-react with NOTCH2 or NOTCH3.
• Formulation: PBS pH 7.4, 50% Glycerol
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium azide
• Concentration: lot specific
• Purification: Protein G chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: notch 1
• Database Link:
  Entrez Gene 18128 Mouse
  UniProt ID Q01705
• Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furinlike convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a Cterminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
• Synonyms: Notch 1, hN1, TAN1