hCG (CGA) Mouse Monoclonal Antibody [Clone ID: HCGa/53]

CAT#: AM50278PU-S

hCG (CGA) mouse monoclonal antibody, clone HCGa/53, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human placenta stained with hCG alpha Antibody (Clone HCGa/53). Note specific membrane staining.

Specifications

Product Data

• Clone Name: HCGa/53
• Applications: IHC, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Western Blotting: 0.5-1 µg/ml.
  Immunohistolochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Placenta.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant hCG alpha protein.
• Specificity: This MAb reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
  Cellular Localization: Cytoplasmic, secreted.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Proten A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~13 kDa
• Gene Name: glycoprotein hormones, alpha polypeptide
• Database Link:
  Entrez Gene 1081 Human
  UniProt ID P01215
• Background: HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
• Synonyms: Follitropin alpha chain, Follicle-stimulating hormone alpha chain, FSH-alpha, Lutropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, LH alpha, Thyrotropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, hCG-alpha