CD6 Mouse Monoclonal Antibody [Clone ID: C6/372]

CAT#: AM50242PU-T

CD6 mouse monoclonal antibody, clone C6/372, Purified

Product Images

Formalin-Paraffin tonsil (10X) stained with CD6 Antibody (Clone C6/372).

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  Formalin-Paraffin tonsil (20X) stained with CD6 Antibody (Clone C6/372).

Specifications

Product Data

• Clone Name: C6/372
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein.
  Western Blotting: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: CCRF-CEM, Jurkat cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant CD6 protein.
• Specificity: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
  Cellular Localization: Cell surface and cytoplasmic
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 90-130 kDa
• Gene Name: CD6 molecule
• Database Link:
  Entrez Gene 923 Human
  UniProt ID P30203
• Synonyms: T12, TP120