Bcl x (BCL2L1) Mouse Monoclonal Antibody [Clone ID: BX006 + 2H12]

CAT#: AM50202PU-T

Bcl x (BCL2L1) mouse monoclonal antibody, clone BX006 + 2H12, Purified

Product Images

Western blot analysis of Jurkat Cell lysate using Bcl-X Antibody (Clone BX006 + 2H12).

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  Formalin-Paraffin Hodgkin's lymphoma stained with Bcl-X Antibody (Clone BX006 + 2H12). Note cytoplasmic and membrane staining.

Specifications

Product Data

• Clone Name: BX006 + 2H12
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tiss ue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.
• Reactivities: Human, Mouse, Porcine, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of Human Bcl-X protein (BX006 & 2H12).
• Specificity: Recognizes a protein of 27kDa, identified as the Bcl-X protein.
  This Monoclonal Antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2.
  This Monoclonal Antibody reacts with both Bcl-XS and Bcl-XL proteins.
  Cellular Localization: Cytoplasmic and cell/nuclear membrane.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 27 kDa
• Gene Name: BCL2 like 1
• Database Link:
  Entrez Gene 598 Human
  UniProt ID Q07817
• Synonyms: Bcl2-L-1, BCL2L1, BCL2L, BCLX, Bcl-x, bcl-xL, bcl-xS, Bcl-2-like protein 1