Mucin 5AC (MUC5AC) Mouse Monoclonal Antibody [Clone ID: SPM297]

CAT#: AM50143PU-S

Mucin 5AC (MUC5AC) mouse monoclonal antibody, clone SPM297, Purified

Product Images

Intracellular flow cytometry of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells using Mucin-5AC Antibody (Clone SPM297) (red) and isotype control (green).

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  Formalin-paraffin human stomach stained with Mucin-5AC Antibody (Clone SPM297).

Specifications

Product Data

• Clone Name: SPM297
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Stomach.
• Reactivities: Chicken, Feline, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient.
• Specificity: This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
  Cellular Localization: Cytoplasmic.
  Negative Species: Bovine.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: > 1,000 kDa
• Gene Name: mucin 5AC, oligomeric mucus/gel-forming
• Database Link:
  Entrez Gene 4586 Human
  UniProt ID P98088
• Synonyms: MUC5AC, MUC5, TBM, LeB