Hsp60 (HSPD1) Mouse Monoclonal Antibody [Clone ID: SPM253]

CAT#: AM50115PU-S

Hsp60 (HSPD1) mouse monoclonal antibody, clone SPM253, Purified

Product Images

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  Formalin-Paraffin Human Pancreas stained with HSP60 Antibody (Clone SPM253).

Specifications

Product Data

• Clone Name: SPM253
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60, Breast carcinoma.
• Reactivities: Bovine, Canine, Chicken, Drosophila, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human HSP60 protein.
• Specificity: Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp’s), more appropriately called as stress response proteins (srp’s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
  Cellular Localization: Mitochondria in cytoplasm.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60 kDa
• Gene Name: heat shock protein family D (Hsp60) member 1
• Database Link:
  Entrez Gene 3329 Human
  UniProt ID P10809
• Synonyms: HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60