LAYN Mouse Monoclonal Antibody [Clone ID: AT20G8]

CAT#: AM50080PU-S

LAYN mouse monoclonal antibody, clone AT20G8, Purified

Product Images

The cell lysates of A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT20G8
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human LAYN (22-235aa) purified from E. coli
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: layilin
• Database Link:
  Entrez Gene 143903 Human
  UniProt ID Q6UX15
• Background: Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.
• Synonyms: LAYN

Reference Data

• Protein Families: Transmembrane