PPM1F Mouse Monoclonal Antibody [Clone ID: AT6D11]

CAT#: AM50063PU-S

PPM1F mouse monoclonal antibody, clone AT6D11, Purified

Product Images

The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT6D11
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human PPM1F (1-454aa) purified from E. coli
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein phosphatase, Mg2+/Mn2+ dependent 1F
• Database Link:
  Entrez Gene 9647 Human
  UniProt ID P49593
• Background: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
• Synonyms: KIAA0015, POPX2, Partner of PIX 2, Protein fem-2 homolog, CaM-kinase phosphatase, CaMKPase

Reference Data

• Protein Families: Druggable Genome, Phosphatase