PFKM Mouse Monoclonal Antibody [Clone ID: AT2F11]

CAT#: AM50026PU-N

PFKM mouse monoclonal antibody, clone AT2F11, Purified

Product Images

The HepG2 cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human PFKM antibody (1/500~1/2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2F11
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/500-1/2000.
  Recommended Sarting Dilution: 1/2000.
  Flow Cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human PFKM (1-780aa) purified from E. coli
• Specificity: This Monoclonal antibody recognizes Human PFKM. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: phosphofructokinase, muscle
• Database Link:
  Entrez Gene 5213 Human
  UniProt ID P08237
• Background: PFKM (phosphofructokinase, muscle) is a regulatory glycolytic enzyme that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.
• Synonyms: Phosphofructokinase-M, Phosphohexokinase, Phosphofructokinase 1, PFK1, PFK-1, PFK-A, PFKX, GSD7

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway