Cyclin B1 (CCNB1) Mouse Monoclonal Antibody [Clone ID: V92.1]

CAT#: AM33367PU-T

Cyclin B1 (CCNB1) mouse monoclonal antibody, clone V92.1, Purified

Specifications

Product Data

• Clone Name: V92.1
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen Sections: 1-2 μg/ml for 30 minutes at RT.
  Positive Control: Any human cell line in logarithmic growth phase, tonsil, or breast carcinoma.
• Reactivities: Hamster, Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Hamster Cyclin B1 protein.
• Specificity: This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 55-62 kDa
• Gene Name: cyclin B1
• Database Link:
  Entrez Gene 891 Human
  UniProt ID P14635
• Background: Cyclins are evolutionarily conserved regulatory proteins that are essential for controlling the progression of the cell-cycle. Proliferating cells express both cyclin B1 and cyclin B2; expression is normally low in G0/G1, increases in S, and is maximal during the G2/M phase of cell division. Cyclin B1 can be found both on intracellular membranes and free in the cytoplasm, in contrast to cyclin B2, which is membrane-associated. Cyclin B1 binds to p34Cdc2, also known as Cdk1, a cyclin-dependent kinase, to form an inactive complex that accumulates in the cytoplasm. Dephosphorylation by Cdc25 results in activation of the complex and translocation to the nucleus. Cyclin B1 is rapidly degraded at the end of mitosis, and this degradation is essential for cells to exit from mitosis.
• Synonyms: CCNB1, CCNB, Cyclin-B1