CD98 (SLC3A2) Mouse Monoclonal Antibody [Clone ID: UM7F8]

CAT#: AM33318PU-S

CD98 (SLC3A2) mouse monoclonal antibody, clone UM7F8, Purified

Specifications

Product Data

• Clone Name: UM7F8
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution:
  Comitogenic with soluble anti-CD2 and immobilized anti-CD3 monoclonal Antibodies.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Positive Control: HL-60, Jurkat, MG63, HUT-78, K562, YT, U937, Hep-G2 cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Molt 13 T cell line.
• Specificity: This Monoclonal Antibody UM7F8 recognizes CD98. The CD98 molecule is a heterodimer made of a disulfide-linked glycosylated heavy chain of approximately 80-90 kDa and a non-glycosylated light chain. The heavy chain is a type II integral membrane protein containing a 50-81 amino acid NH2-terminal cytoplasmic domain, a single transmembrane sequence, and a large extracellular domain. CD98 is detected on monocytes and activated cells.
  Cellular Localization: Cell surface, cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: Heterodimer (125 kDa) made of a disulfide-linked glycosylated heavy chain of approximately 80-90 kDa and a non-glycosylated light chain
• Gene Name: solute carrier family 3 member 2
• Database Link:
  Entrez Gene 6520 Human
  UniProt ID P08195
• Background: CD98 exits as a heterodimer containing a disulphide-linked glyc osylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exist s as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulatio n of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.
• Synonyms: 4F2hc, MDU1