Prolactin Receptor (PRLR) Mouse Monoclonal Antibody [Clone ID: B6.2+PRLR742]

CAT#: AM33296PU-S

Prolactin Receptor (PRLR) mouse monoclonal antibody, clone B6.2+PRLR742, Purified

Specifications

Product Data

• Clone Name: B6.2+PRLR742
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.25-0.5 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
  Positive Control: Breast, placenta, liver or pancreas.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Semi-purified Human Prolactin Receptor (B6.2) and Recombinant Human Prolactin Receptor (PRLR742).
• Specificity: It recognizes a protein of 70kDa, identified as Prolactin Receptor.
  Cellular Localization: Cell surface and cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 70 kDa
• Gene Name: prolactin receptor
• Database Link:
  Entrez Gene 5618 Human
  UniProt ID P16471
• Background: Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.
• Synonyms: PRLR, PRL-R