Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: SPM537]

CAT#: AM33288PU-T

Bromodeoxyuridine / BrDU mouse monoclonal antibody, clone SPM537, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Mouse intestine tissue stained with BrdU Antibody Cat.-No AM33288PU (Clone SPM537).

Specifications

Product Data

• Clone Name: SPM537
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.
  For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37°C.
  Recommended Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH
• Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
  Cellular Localization: Nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: Depends on the target
• Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.