Dibromo-Tyrosine (DiBrY) Mouse Monoclonal Antibody [Clone ID: 3A5]

CAT#: AM33191PU-N

Dibromo-Tyrosine (DiBrY) mouse monoclonal antibody, clone 3A5, Purified

Product Images

Immunohistochemistry of Dibromo-tyrosine staining in Paraffin Sections of LPS-treated Mouse Liver. Antigen retrieval was performed by Microwave treatment in 0.01M Citrate buffer (pH 6.0) for 5 min. The sections were incubated with AM33191PU (10 ug/ml) and detected using Biotinylated secondary antibody and Vectastain ABC-AP Kit. A: Liver section of untreated Mouse. B: Liver section of LPS-treated Mouse sacrificed after 24h. C: Liver section of LPS-treated Mouse sacrificed after 72h. Kato Y et al (2005) Free Radic Biol Med 38 (1): 24-31.

Specifications

Product Data

• Clone Name: 3A5
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA (Ref.1).
  Western blot.
  immunohistochemistry on Paraffin Sections: 10 μg/ml (Ref.1).
  
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 3,5-Dibromo-Tyrosine KLH-conjugated.
• Specificity: Specific for di-halogenated Tyrosine. Recognizes Dibromo-Tyrosine (DiBrY), N-acetylated dichlorotyrosine (NAcDiClY), diiodo-tyrosine (DiIY), N-bromo-succinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosine conjugated BSA. Bromotyrosine (BrY) is weakly recognized.
  There was no cross reactivity obtained with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chlorotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3, 4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. No cross reactivity was obtained with brominated nucleic acids 5-bromouracil, 5-bromouridine, and 8-bromoguanosine.
• Formulation: 10 mM PBS pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 200 µl aqua bidest to 0.1 mg/ml
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: Diamine oxidase, AOC1, DAO1, Amiloride-binding protein, Histaminase, Kidney amine oxidase