VCAM1 Mouse Monoclonal Antibody [Clone ID: 4B9]

CAT#: AM33018PU-N

VCAM1 mouse monoclonal antibody, clone 4B9, Purified

Specifications

Product Data

• Clone Name: 4B9
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Western blot.
  Flow Cytometry.
  Immunoprecipitation.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections.
  Recommended Dilutions: Use 1/25–1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Immunization of Balb/c mice with human umbilical vein endothelium (HUVE) that were nonenzymatically harvested after stimulation for 24h with recombinant human (rh) TNF-α (10 ng/ml).
  Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.
• Specificity: The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: vascular cell adhesion molecule 1
• Database Link:
  Entrez Gene 7412 Human
  UniProt ID P19320
• Background: Vascular cell adhesion molecule-1 (VCAM-1) is induced on human umbilical vein endothelium (HUVE) by recombinant human tumor necrosis factor - α (rh TNF-α), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigration at sites of inflammation and immune reaction.
• Synonyms: V-CAM 1, INCAM-100, L1CAM, VCAM-1