Actin (ACTA1) Mouse Monoclonal Antibody [Clone ID: 3B3]

CAT#: AM33000PU-N

Actin (ACTA1) mouse monoclonal antibody, clone 3B3, Purified

Product Images

Paraffin sections of human striated skeletal muscle from human tongue immunostained with 3B3 (1/250)

• 

  Paraffin sections of striated muscle from human tongue immunostained with 3B3 (1/250)

Specifications

Product Data

• Clone Name: 3B3
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Flow Cytometry.
  Immunoblotting.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin-Embedded Sections.
  Recommended Dilutions: 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
• Reactivities: Human, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide comprising the N-terminal nonapeptide of α-skeletal Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.
• Specificity: This 3B3 Monoclonal antibody is highly specific for Alpha-Skeletal Actin, and does not cross react with other Actin isoforms.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: actin, alpha 1, skeletal muscle
• Database Link:
  Entrez Gene 58 Human
  UniProt ID P68133
• Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates.
  Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.
• Synonyms: ACTA; Alpha-actin-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3