S100A1 Mouse Monoclonal Antibody [Clone ID: 4C4.9]

CAT#: AM32830PU-T

S100A1 mouse monoclonal antibody, clone 4C4.9, Purified

Product Images

Western blot analysis of Human brain lysate using S100 Antibody at 1 ug/ml.

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  Formalin-Fixed, Paraffin-Embedded Human melanoma stained with S100 Antibody (Clone 4C4.9).

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  Formalin-Fixed, Paraffin-Embedded Human melanoma stained with S100 Antibody (Clone 4C4.9).

Specifications

Product Data

• Clone Name: 4C4.9
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use C4.9 antibody at 0.25-0.5 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 1mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Recommended Positive Control: Brain, Melanoma.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: S100 protein purified from Bovine brain.
• Specificity: This Monoclonal antibody stains S100 protein.
  4C4.9 stains Schwannomas, ependymomas, astrogliomas; almost all benign and malignant melanomas and their metastases.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 10-12 kDa
• Gene Name: S100 calcium binding protein A1
• Database Link:
  Entrez Gene 6271 Human
  UniProt ID P23297
• Background: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas and their metastases.
• Synonyms: S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain, S-100 protein beta chain, Astrocyte Marker