CD41-CD61 Complex Mouse Monoclonal Antibody [Clone ID: Y8]

CAT#: AM32633PU-N

CD41-CD61 Complex mouse monoclonal antibody, clone Y8, Aff - Purified

Specifications

Product Data

• Clone Name: Y8
• Applications: ELISA, WB
• Recommended Dilution: This GPIIb/IIIa antibody is Suitable for use in ELISA and Western Blot.
  Use of this MAb at a concentration of 10-20 µg/ml (culture supernatants) will allow visualization of NP 40 solubilized human normal platelets.
  Under reducing conditions they will bind against the human platelet membrane glycoproteins IIb/IIIa complex, which have a molecular weights of ~130,000 (IIb) and 115,000 (IIIa).
  The titer of purified ascites was determined by ELISA coated with 100 µl of 1x10⁸ washed platelets.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 10e9 Human platelets suspension. The supernatant fluid from positive clones was screened by ELISA coated with washed platelets.
• Specificity: Human platelet membrane glycoproteins IIb/IIIa (GP IIb/IIIa).
• Formulation: 0.01M PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: The integrins are a family of more than 23 heterodimeric transmembrane proteins that mediate cell-cell adhesions as well as cell-substratum adhesions and signal transduction processes. Integrins are heterodimers consisting of noncovalently associated alpha and beta subunits. CD61 non-covalently associates with CD41 (alpha Ilb integrin) and is expressed by megakaryocytes and platelets. The CD61/CD41 complex acts as a receptor for such adhesive ligands as fibronectin, fibrinogen and von Willebrand factor during platelet stimulation.
• Synonyms: GPIIb-IIIa, ITGA2B, GP2B, ITGAB, Platelet membrane glycoprotein IIb, GPalpha IIb, GPIIb, GP3A, CD41, CD61, Integrin alpha-IIb, Integrin beta-3, ITGB3